Nitrogen fixing is really, really hard work. It does cost plant quite a lot of energy to feed and protect the rhizobium in the roots. There is a net benefit to the plant but there's no such thing as a free l;Wisteria can fix Nitrogen so most likely root nodules that fix N? That is why I try to stay away from feeding mine (not a bonsai though) any fertilizer that has N. Still hasn't flowered in 15 years so I'm kinda over it, tried root pruning, hard pruning, etc. I'm done with that sumbatch![]()
if they are nodules, you can split one open with your finger nail. If it is pink inside, it is active fixing nitrogen.Here is a picture of some of the pruned roots.
Not all individual legumes get nodules. There needs to be some rhizobium bacteria in the soil to invade the roots. Then the plant builds a house for the bacteria - little nodules. None of the correct bacteria no nodules.
Farmers get legume seeds coated in the correct strains for the plants they are sowing so the bacteria are right there when the seed germinates. I have no idea whether commercial strains would be compatible with wisteria. Most legumes can associate with a range of different bacterial strains but some are more effective than others. If you wanted to get nodules you could try adding a commercial source of rhizobia and see if that helps or introduce some soil or roots from a healthy wisteria and see what happens.
As mentioned earlier these plants can live quite happily without rhizobia as long as they get regular fertilizer. The bacterial association is just an adaptation that helps the plants compete when nutrient levels are a bit low.
Wisteria roots nodules from wikipedia: https://commons.wikimedia.org/wiki/File:Soil_fertility_-_nitrogen_fixation_by_root_nodules_on_Wistaria_roots,_with_hazelnut_to_show_size.JPG
But havent seen any root nodules on my wisteria at repoting.