PGR Harmones in Scot pine

[Nishant]

Hello All,

I know that Auxin, cytokinin and gibberellin are the three plant growth regulator hormones. Is it true that for each species the actual chemical compound is different? And if so what are the chemical compounds of scot pine?

Thanks.

 

[Wires_Guy_wires]

Auxin, cytokinin and gibberillin are umbrella terms.
It's difficult to say which ones are the chemical compounds for scots pines because most of them have been tested and most of them had an effect.
If you want to do a technique I think it might be wise to just check google scholar and see whatever was used in a study that did the same thing as you want to do.

For instance: vegetative reproduction of scots pines will yield you studies that used NAA, IBA and IAA as auxins, and probably 6-bap as a cytokinin.

 

[Nishant]

Many Thanks guy_wire.

I am trying to speed up the callus formation so as to get a better success rate with grafting.

So my idea that approach grafting takes a while to and is also failed by the sap flowing into the union from both stock and scion branches.

So I was thinking of letting the cuts heal on their own while callus forms. So basically just apply callus inducing harmone, wrap it in moist environment like done for air layering. Any sap that flows in can be wiped off periodically. Soon the sap flow will stop and if the callus harmone worked, a fair amount of callus would form. At this stage, splice together the branches.

This technique will reduce the need to position the cambium exactly over each other. I believe the callus formed separately will fuse much easily. So this method can help make approach graft doable even in peak summer when sap is out to destroy any grafting work done.

What say?

 

[Wires_Guy_wires]

It's something I have been wanting to try myself. But the key issue is that callus tissue has no functional sap flow by itself and is being kept alive by sap that's seeping through it: diffusion.
So I'm not sure if the callus tissue itself would improve your chances, but it is worth a shot!

6-benzylaminopurine as well as zeatin can be used to increase callus growth. So applying one of those at high concentrations (a piece of cotton soaked in a 1mg/L solution) might work. But again, I'm not sure if the sap flow, that feeds the callus, would be enough to feed a scion.

 

[Nishant]

Many Thanks for encouragement. I will give it a try and tell how it goes.